Rabbit anti-Human, Mouse BCL7B Polyclonal Antibody | anti-BCL7B antibody

BCL7B, CT (BCL7B, B Cell CLL/lymphoma 7 protein family member B, Allergen=Hom s 3) (Azide free) (HRP)

Cat. Num. AAA6278025

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCL7B; Polyclonal Antibody; CT (BCL7B; B Cell CLL/lymphoma 7 protein family member B; Allergen=Hom s 3) (Azide free) (HRP); B-cell CLL/lymphoma 7 protein family member B; Allergen=Hom s 3; anti-BCL7B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BCL7B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BCL7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the C-terminal region of human BCL7B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BCL7B Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the BCL7B antibody detected the BCL7B protein (arrow).)

Western Blot (WB)

(BCL7B Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the BCL7B antibody detected the BCL7B protein (arrow).)

Immunohistochemistry (IHC)

(BCL7B Antibody (C-term) (AP17168b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BCL7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BCL7B antibody

This gene encodes a member of the BCL7 family including BCL7A, BCL7B and BCL7C proteins. This member is BCL7B, which contains a region that is highly similar to the N-terminal segment of BCL7A or BCL7C proteins. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. This gene is highly conserved from C. elegans to human. Multiple alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-BCL7B antibody

Antibodies; Cancer Markers

References

Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010). Kathiresan, S., et al. Nat. Genet. 40(2):189-197(2008). Willer, C.J., et al. Nat. Genet. 40(2):161-169(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006). Jadayel, D.M., et al. Gene 224 (1-2), 35-44 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 20336473
• NCBI GeneID: 9275
• NCBI Accession #: NP_001698.2
• NCBI GenBank Nucleotide #: NM_001707.3
• UniProt Accession #: Q9BQE9; O43769; Q13845; Q6ZW75; A8K226; C9JWD3; D3DXF0
• Molecular Weight: 16,045 Da
• NCBI Official Full Name: B-cell CLL/lymphoma 7 protein family member B isoform 1
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 7B
• NCBI Official Symbol: BCL7B
• NCBI Protein Information: B-cell CLL/lymphoma 7 protein family member B
• UniProt Protein Name: B-cell CLL/lymphoma 7 protein family member B
• Protein Family: B-cell CLL/lymphoma 7 protein family
• UniProt Gene Name: BCL7B
• UniProt Entry Name: BCL7B_HUMAN

NCBI Description

This gene encodes a member of the BCL7 family including BCL7A, BCL7B and BCL7C proteins. This member is BCL7B, which contains a region that is highly similar to the N-terminal segment of BCL7A or BCL7C proteins. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. This gene is highly conserved from C. elegans to human. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

Function: May play a role in lung tumor development or progression.

Tissue specificity: Ubiquitous. Ref.2 Ref.3

Involvement in disease: BCL7B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BCL7B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Ref.2 Ref.3

Allergenic properties: Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations. Ref.9

Sequence similarities: Belongs to the BCL7 family.