Mouse E3 ubiquitin-protein ligase RAD18 (RAD18) ELISA Kit | RAD18 elisa kit

Mouse E3 ubiquitin-protein ligase RAD18 (RAD18) ELISA Kit

Cat. Num. AAA756399

• Gene Names: RAD18; RNF73
• Reactivity: Mouse
• Synonyms: E3 ubiquitin-protein ligase RAD18 (RAD18); Mouse E3 ubiquitin-protein ligase RAD18 (RAD18) ELISA Kit; RAD18 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1ng/mL.
• Intended Uses: This RAD18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RAD18. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAD18 elisa kit

Principle of the Assay: RAD18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAD18 antibody and an RAD18-HRP conjugate. The assay sample and buffer are incubated together with RAD18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAD18 concentration since RAD18 from samples and RAD18-HRP conjugate compete for the anti-RAD18 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAD18 from the sample, fewer sites are left to bind RAD18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAD18 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 256818821
• NCBI GeneID: 56852
• NCBI Accession #: NP_064550.3
• NCBI GenBank Nucleotide #: NM_020165.3
• Molecular Weight: 56,223 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RAD18
• NCBI Official Synonym Full Names: RAD18 E3 ubiquitin protein ligase
• NCBI Official Symbol: RAD18
• NCBI Official Synonym Symbols: RNF73
• NCBI Protein Information: E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18 homolog; RING finger protein 73; RAD18, S. cerevisiae, homolog; postreplication repair protein RAD18; postreplication repair protein hRAD18p
• UniProt Protein Name: E3 ubiquitin-protein ligase RAD18
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RAD18
• UniProt Synonym Gene Names: RNF73; hHR18; hRAD18
• UniProt Entry Name: RAD18_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

Uniprot Description

RAD18: a structural maintenance of chromosomes (SMC) protein involved in postreplication repair of UV-damaged DNA. Has ssDNA binding activity. Interacts with Rad6 through a conserved ring-finger motif. Rad6 is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA.

Protein type: EC 6.3.2.19; DNA repair, damage; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: XY body; replication fork; chromatin; nucleus

Molecular Function: Y-form DNA binding; protein binding; zinc ion binding; ubiquitin protein ligase binding; damaged DNA binding; polyubiquitin binding; ligase activity

Biological Process: negative regulation of DNA recombination; protein ubiquitination; spermatogenesis; DNA repair; response to UV