Human Foxj1 (Forkhead box protein J1) ELISA Kit | Foxj1 elisa kit

Human Foxj1 (Forkhead box protein J1) ELISA Kit

Cat. Num. AAA7606699

• Gene Names: FOXJ1; HFH4; HFH-4; FKHL13
• Reactivity: Human
• Synonyms: Foxj1 (Forkhead box protein J1); Human Foxj1 (Forkhead box protein J1) ELISA Kit; Foxj1; FKHL13; HFH-4; HFH4; fork head homologue 4; forkhead transcription factor HFH-4; forkhead-like 13; forkhead-related protein FKHL13; hepatocyte nuclear factor 3 forkhead homolog 4; forkhead box J1; Foxj1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Foxj1. No significant cross-reactivity or interference between Foxj1 and analogues was observed.
• Sequence Length: 421

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Foxj1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 50301236
• NCBI GeneID: 2302
• NCBI Accession #: NP_001445.2
• NCBI GenBank Nucleotide #: NM_001454.3
• UniProt Accession #: Q92949; O00630
• Molecular Weight: 45,247 Da
• NCBI Official Full Name: forkhead box protein J1
• NCBI Official Synonym Full Names: forkhead box J1
• NCBI Official Symbol: FOXJ1
• NCBI Official Synonym Symbols: HFH4; HFH-4; FKHL13
• NCBI Protein Information: forkhead box protein J1
• UniProt Protein Name: Forkhead box protein J1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXJ1
• UniProt Synonym Gene Names: HFH-4

NCBI Description

This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.[provided by RefSeq, Sep 2009]

Uniprot Description

FOXJ1: May play an important role in cell fate determination during lung development and in spermatogenesis. Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: actin cytoskeleton organization and biogenesis; brain development; central tolerance induction; establishment of apical/basal cell polarity; humoral immune response; inhibition of NF-kappaB transcription factor; leukocyte migration; negative regulation of B cell activation; negative regulation of germinal center formation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of interleukin-6 biosynthetic process; negative regulation of T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; positive regulation of central B cell tolerance induction; positive regulation of transcription from RNA polymerase II promoter

Disease: Allergic Rhinitis