Human 2, 5-OAS (2, 5-oligoadenylate synthetase) ELISA Kit | 2, 5-OAS elisa kit

Human 2, 5-OAS (2, 5-oligoadenylate synthetase) ELISA Kit

Cat. Num. AAA7606307

• Gene Names: OAS3; p100; p100OAS
• Reactivity: Human
• Synonyms: 2; 5-OAS (2; 5-oligoadenylate synthetase); Human 2; 5-oligoadenylate synthetase) ELISA Kit; 5-OAS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of 2, 5-OAS. No significant cross-reactivity or interference between 2, 5-OAS and analogues was observed.
• Sequence Length: 1087

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.469-30ng/ml
• Sensitivity: <0.281ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2, 5- OAS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2, 5- OAS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for 2, 5-OAS elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- 2, 5- OAS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- 2, 5-OAS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 2, 5-OAS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of 2, 5-OAS can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 45007007
• NCBI GeneID: 4940
• NCBI Accession #: NP_006178.2
• NCBI GenBank Nucleotide #: NM_006187.3
• UniProt Accession #: Q9Y6K5; Q2HJ14; Q9H3P5
• Molecular Weight: 121,170 Da
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 3
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 3
• NCBI Official Symbol: OAS3
• NCBI Official Synonym Symbols: p100; p100OAS
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 3
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 3
• UniProt Gene Name: OAS3
• UniProt Synonym Gene Names: (2-5')oligo(A) synthase 3; 2-5A synthase 3; p100OAS

NCBI Description

This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

OAS3: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'- oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV). Belongs to the 2-5A synthase family.

Protein type: EC 2.7.7.84; Transferase

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cytoplasm; cytosol; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: 2'-5'-oligoadenylate synthetase activity; ATP binding; double-stranded RNA binding; protein binding

Biological Process: defense response to virus; negative regulation of viral genome replication; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to virus