Human 2',5'-Oligoadenylate Synthetase 3 (OAS3) RTU ELISA Kit | OAS3 rtu elisa kit

Human 2',5'-Oligoadenylate Synthetase 3 (OAS3) ELISA Kit

Cat. Num. AAA4501537

• Gene Names: OAS3; p100; p100OAS
• Reactivity: Human
• Synonyms: 2'; 5'-Oligoadenylate Synthetase 3 (OAS3); Human 2'; 5'-Oligoadenylate Synthetase 3 (OAS3) ELISA Kit; p100OAS; OAS3 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of OAS3. No significant cross-reactivity or interference between OAS3 and analogues was observed.
• Sequence Length: 1087

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.27ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OAS3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OAS3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OAS3 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of OAS3 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OAS3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OAS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OAS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OAS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45007007
• NCBI GeneID: 4940
• NCBI Accession #: NP_006178.2
• NCBI GenBank Nucleotide #: NM_006187.3
• UniProt Accession #: Q9Y6K5; Q2HJ14; Q9H3P5
• Molecular Weight: 121,170 Da
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 3
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 3
• NCBI Official Symbol: OAS3
• NCBI Official Synonym Symbols: p100; p100OAS
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 3
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 3
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS3
• UniProt Synonym Gene Names: (2-5')oligo(A) synthase 3; 2-5A synthase 3; p100OAS

NCBI Description

This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).