Human 2'-5'-oligoadenylate synthase 3 ELISA Kit | OAS3 elisa kit

Human 2'-5'-oligoadenylate synthase 3 ELISA Kit

Cat. Num. AAA760140

• Gene Names: OAS3; p100; p100OAS
• Reactivity: Human
• Synonyms: 2'-5'-oligoadenylate synthase 3; Human 2'-5'-oligoadenylate synthase 3 ELISA Kit; OAS3/2'-5'-oligoadenylate synthase 3/ (2-5')oligo (A) synthase 3/2-5A synthase 3/p100 OAS/p100OAS/P/OKcl.4; OAS3 elisa kit

• Reactivity: Human
• Sequence Length: 1087

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for OAS3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OAS3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OAS3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OAS3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OAS3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 45007007
• NCBI GeneID: 4940
• NCBI Accession #: NP_006178.2
• NCBI GenBank Nucleotide #: NM_006187.3
• Molecular Weight: 121,170 Da
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 3
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 3
• NCBI Official Symbol: OAS3
• NCBI Official Synonym Symbols: p100; p100OAS
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 3
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 3
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS3
• UniProt Synonym Gene Names: (2-5')oligo(A) synthase 3; 2-5A synthase 3; p100OAS
• UniProt Entry Name: OAS3_HUMAN

NCBI Description

This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

OAS3: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'- oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV). Belongs to the 2-5A synthase family.

Protein type: EC 2.7.7.84; Transferase

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: cytoplasm; cytosol; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: 2'-5'-oligoadenylate synthetase activity; ATP binding; double-stranded RNA binding; metal ion binding; protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; negative regulation of viral genome replication; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to virus