Human Alpha-tocopherol transfer protein ELISA Kit | TTPA elisa kit

Human Alpha-tocopherol transfer protein ELISA Kit

Cat. Num. AAA760491

• Gene Names: TTPA; ATTP; AVED; TTP1; alphaTTP
• Reactivity: Human
• Synonyms: Alpha-tocopherol transfer protein; Human Alpha-tocopherol transfer protein ELISA Kit; TTPA/Alpha-tocopherol transfer protein/Alpha-TTP/TPP1; TTPA elisa kit

• Reactivity: Human
• Sequence Length: 278

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TTPA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TTPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TTPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TTPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TTPA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507723
• NCBI GeneID: 7274
• NCBI Accession #: NP_000361.1
• NCBI GenBank Nucleotide #: NM_000370.3
• Molecular Weight: 31,750 Da
• NCBI Official Full Name: alpha-tocopherol transfer protein
• NCBI Official Synonym Full Names: tocopherol (alpha) transfer protein
• NCBI Official Symbol: TTPA
• NCBI Official Synonym Symbols: ATTP; AVED; TTP1; alphaTTP
• NCBI Protein Information: alpha-tocopherol transfer protein
• UniProt Protein Name: Alpha-tocopherol transfer protein
• Protein Family: Alpha-tocopherol transfer protein
• UniProt Gene Name: TTPA
• UniProt Synonym Gene Names: TPP1; Alpha-TTP
• UniProt Entry Name: TTPA_HUMAN

NCBI Description

This gene encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. [provided by RefSeq, Nov 2009]

Uniprot Description

TTPA: Binds alpha-tocopherol and enhances its transfer between separate membranes. Defects in TTPA are the cause of ataxia with isolated vitamin E deficiency (AVED). AVED is an autosomal recessive disease characterized by spinocerebellar degeneration. It causes ataxia and peripheral neuropathy that resembles Friedreich ataxia. AVED patients have markedly reduced plasma levels of vitamin E.

Chromosomal Location of Human Ortholog: 8q12.3

Cellular Component: cytosol; late endosome

Molecular Function: phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; transporter activity; vitamin E binding

Biological Process: embryonic placenta development; intermembrane transport; intracellular pH reduction; lipid metabolic process; response to nutrient; response to pH; response to toxin; transport; vitamin E metabolic process; vitamin transport

Disease: Vitamin E, Familial Isolated Deficiency Of