Western Blot (WB)
(PR38A Antibody (C-term) western blot analysis in MDA-MB231,CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the PR38A antibody detected the PR38A protein (arrow).)
Rabbit PR38A Polyclonal Antibody | anti-PRPF38A antibody
PR38A Antibody (C-term)
Gene Names
PRPF38A; Prp38
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Xenopus)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PR38A; Polyclonal Antibody; PR38A Antibody (C-term); Pre-mRNA-splicing factor 38A; PRPF38A; anti-PRPF38A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PR38A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-312 amino acids from the C-terminal region of human PR38A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
283-312
Sequence Length
125
Applicable Applications for anti-PRPF38A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PR38A Antibody (C-term) western blot analysis in MDA-MB231,CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the PR38A antibody detected the PR38A protein (arrow).)
Western Blot (WB)
(PR38A Antibody (C-term) western blot analysis in MDA-MB231,CEM,HL-60 cell line lysates (35ug/lane).This demonstrates the PR38A antibody detected the PR38A protein (arrow).)
Immunohistochemistry (IHC)
(PR38A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PR38A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PR38A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PR38A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PR38A Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PR38A Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
References
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37477
NCBI Official Full Name
pre-mRNA-splicing factor 38A
NCBI Official Synonym Full Names
pre-mRNA processing factor 38A
NCBI Official Symbol
PRPF38A
NCBI Official Synonym Symbols
Prp38
NCBI Protein Information
pre-mRNA-splicing factor 38A
UniProt Protein Name
Pre-mRNA-splicing factor 38A
Protein Family
UniProt Gene Name
PRPF38A
UniProt Entry Name
PR38A_HUMAN
Uniprot Description
PRPF38A: May be required for pre-mRNA splicing (Potential). Belongs to the PRP38 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing
Chromosomal Location of Human Ortholog: 1p32.3
Cellular Component: nuclear membrane; nucleus
Molecular Function: protein binding
Biological Process: RNA splicing; mRNA processing
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Product Notes
The PRPF38A prpf38a (Catalog #AAA9202046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 283-312. The PR38A Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's PR38A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PRPF38A prpf38a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PR38A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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