Bovine Prolactin ELISA Kit | PRL elisa kit

Bovine Prolactin ELISA Kit

Cat. Num. AAA760177

• Gene Names: PRL; GHA1
• Reactivity: Bovine
• Synonyms: Prolactin; Bovine Prolactin ELISA Kit; PRL elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRL. No significant cross-reactivity or interference between PRL and analogues was observed.
• Sequence Length: 227

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRL elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 254675133
• NCBI GeneID: 5617
• NCBI Accession #: NP_001157030.1
• NCBI GenBank Nucleotide #: NM_001163558.2
• Molecular Weight: 25,876 Da
• NCBI Official Full Name: prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Official Synonym Symbols: GHA1
• NCBI Protein Information: prolactin
• UniProt Protein Name: Prolactin
• Protein Family: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region; intracellular

Molecular Function: hormone activity; prolactin receptor binding; protein binding

Biological Process: cell proliferation; cell surface receptor linked signal transduction; cellular protein metabolic process; female pregnancy; lactation; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth