Porcine Glucocorticoid receptor ELISA Kit | GR elisa kit

Porcine Glucocorticoid receptor ELISA Kit

Cat. Num. AAA752876

• Gene Names: NR3C1; GR; GCR; GRL; GCCR; GCRST
• Reactivity: Porcine
• Synonyms: Glucocorticoid receptor; Porcine Glucocorticoid receptor ELISA Kit; GR elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GR. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GR elisa kit

Principle of the Assay: GR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GR antibody and an GR-HRP conjugate. The assay sample and buffer are incubated together with GR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GR concentration since GR from samples and GR-HRP conjugate compete for the anti-GR antibody binding site. Since the number of sites is limited, as more sites are occupied by GR from the sample, fewer sites are left to bind GR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 239758
• NCBI GeneID: 2908
• Molecular Weight: 82,904 Da
• NCBI Official Full Name: glucocorticoid receptor, partial
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• NCBI Official Symbol: NR3C1
• NCBI Official Synonym Symbols: GR; GCR; GRL; GCCR; GCRST
• NCBI Protein Information: glucocorticoid receptor; glucocorticoid nuclear receptor variant 1
• UniProt Protein Name: Glucocorticoid receptor
• Protein Family: Glucocorticoid receptor
• UniProt Gene Name: NR3C1
• UniProt Synonym Gene Names: GRL; GR
• UniProt Entry Name: GCR_HUMAN

NCBI Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Uniprot Description

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.

Protein type: DNA-binding; Mitochondrial; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; glucocorticoid receptor activity; zinc ion binding; transcription factor activity; steroid binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; positive regulation of neuron apoptosis; regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression

Disease: Glucocorticoid Resistance, Generalized