Porcine Acetyl Coenzyme A Carboxylase Alpha ELISA Kit | ACACa elisa kit

Porcine Acetyl Coenzyme A Carboxylase Alpha ELISA Kit

Cat. Num. AAA751112

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Porcine
• Synonyms: Acetyl Coenzyme A Carboxylase Alpha; Porcine Acetyl Coenzyme A Carboxylase Alpha ELISA Kit; ACACa elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACACa. No significant cross-reactivity or interference between ACACa and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACACa and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACACa elisa kit

Intended Uses: This ACACa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ACACa. This ELISA kit for research use only!

Principle of the Assay: ACACa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACACa antibody and an ACACa-HRP conjugate. The assay sample and buffer are incubated together with ACACa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACACa concentration since ACACa from samples and ACACa-HRP conjugate compete for the anti-ACACa antibody binding site. Since the number of sites is limited, as more sites are occupied by ACACa from the sample, fewer sites are left to bind ACACa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACACa concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38679977
• NCBI GeneID: 31
• NCBI Accession #: NP_942136.1
• NCBI GenBank Nucleotide #: NM_198839.2
• Molecular Weight: 269,999 Da
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 2
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1; ACC-alpha; acetyl-Coenzyme A carboxylase alpha
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1
• UniProt Entry Name: ACACA_HUMAN

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Lipid Metabolism - fatty acid biosynthesis; Ligase; Carbohydrate Metabolism - propanoate; EC 6.4.1.2; EC 6.3.4.14; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; positive regulation of cellular metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; carnitine shuttle; cellular lipid metabolic process; biotin metabolic process; protein homotetramerization; multicellular organismal protein metabolic process; energy reserve metabolic process; tissue homeostasis; fatty acid biosynthetic process; water-soluble vitamin metabolic process

Disease: Acetyl-coa Carboxylase Deficiency