Mouse anti-Human WDR5 Monoclonal Antibody | anti-WDR5 antibody

WDR5 Antibody

Cat. Num. AAA9602687

• Gene Names: WDR5; SWD3; BIG-3; CFAP89
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-Chromatography
• Synonyms: WDR5; Monoclonal Antibody; WDR5 Antibody; 2410008O07Rik; AA408785; AA960360; BIG 3; Big; BIG3; BMP2 induced 3 kb gene protein; BMP2 induced gene; 3-KB; BMP2-induced 3-kb gene protein; MGC114572; OTTHUMP00000162494; SWD 3; SWD3; SWD3 Set1c WD40 repeat protein homolog; WD repeat containing protein 5; WD repeat domain 5; WD repeat protein 5; WD repeat-containing protein 5; WD repeat-containing protein BIG-3; WDR 5; Wdr5; WDR5_HUMAN; anti-WDR5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: WDR5 antibody detects endogenous levels of total WDR5
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 334

• Applicable Applications for anti-WDR5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000
• Immunogen: Purified recombinant fragment of human WDR5 expressed in E. Coli
• Subcellular Location: Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using WDR5 mouse mAb against Hela (1) cell lysate.)

Related Product Information for anti-WDR5 antibody

Description: WD-repeat protein 5 (WDR5, also designated BMP-2-induced gene 3 kb or BIG-3) belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. WDR5 is a conserved subunit of Trithorax (TRX) histone methyltransferase complexes that selectively binds to dimethylated Lys4 (K4me2) in Histone H3 to promote K4 trimethylation by TRX. It is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. The WDR5 protein contains 7 WD-repeats, which may play a role in its function of accelerating osteoblast differentiation.
Function: Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.
Subunit Structure: Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3. Interacts with HCFC1. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha-and beta-tubulin. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KMT2A/MLL1 and RBBP5; the interaction is direct. Component ofthe ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and KAT14. Interacts with histone H3. Interacts with SETD1A. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Interacts with PER1.
Similarity: Belongs to the WD repeat WDR5/wds family.

NCBI and Uniprot Product Information

• NCBI GI #: 16554627
• NCBI GeneID: 11091
• NCBI Accession #: NP_060058.1
• NCBI GenBank Nucleotide #: NM_017588.2
• UniProt Accession #: P61964; Q91VA5; Q9NWX7; Q9UGP9
• Molecular Weight: Observed: 36.6 kDa; Predicted: 37 kDa
• NCBI Official Full Name: WD repeat-containing protein 5
• NCBI Official Synonym Full Names: WD repeat domain 5
• NCBI Official Symbol: WDR5
• NCBI Official Synonym Symbols: SWD3; BIG-3; CFAP89
• NCBI Protein Information: WD repeat-containing protein 5
• UniProt Protein Name: WD repeat-containing protein 5
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR5
• UniProt Synonym Gene Names: BIG3

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.