Bovine Microtubule-associated protein 1B ELISA Kit | MAP1B elisa kit

Bovine Microtubule-associated protein 1B ELISA Kit

Cat. Num. AAA749171

• Gene Names: MAP1B; MAP5; FUTSCH; PPP1R102
• Reactivity: Bovine
• Synonyms: Microtubule-associated protein 1B; Bovine Microtubule-associated protein 1B ELISA Kit; MAP1B elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAP1B. No significant cross-reactivity or interference between MAP1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAP1B and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAP1B elisa kit

Intended Uses: This MAP1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of BovineMAP1B. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: MAP1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MAP1B antibody and an MAP1B-HRP conjugate. The assay sample and buffer are incubated together with MAP1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAP1B concentration since MAP1B from samples and MAP1B-HRP conjugate compete for the anti-MAP1B antibody binding site. Since the number of sites is limited, as more sites are occupied by MAP1B from the sample, fewer sites are left to bind MAP1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAP1B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MAP1B elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 153945728
• NCBI GeneID: 4131
• NCBI Accession #: NP_005900.2
• NCBI GenBank Nucleotide #: NM_005909.3
• Molecular Weight: 270,634 Da
• NCBI Official Full Name: microtubule-associated protein 1B
• NCBI Official Synonym Full Names: microtubule-associated protein 1B
• NCBI Official Symbol: MAP1B
• NCBI Official Synonym Symbols: MAP5; FUTSCH; PPP1R102
• NCBI Protein Information: microtubule-associated protein 1B; MAP-1B; protein phosphatase 1, regulatory subunit 102
• UniProt Protein Name: Microtubule-associated protein 1B
• Protein Family: Methionine aminopeptidase
• UniProt Gene Name: MAP1B
• UniProt Synonym Gene Names: MAP-1B
• UniProt Entry Name: MAP1B_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

MAP1B: the function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: microtubule; microtubule associated complex; cytoplasm; postsynaptic density; plasma membrane; dendritic spine; cell junction; cytosol

Molecular Function: protein binding; microtubule binding; structural molecule activity

Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of axon extension; dendrite development; cellular process; microtubule bundle formation; mitochondrion transport along microtubule