Sheep Tenascin ELISA Kit | TN elisa kit

Sheep Tenascin ELISA Kit

Cat. Num. AAA745323

• Gene Names: TNC; GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• Reactivity: Sheep
• Synonyms: Tenascin; Sheep Tenascin ELISA Kit; TN elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This Tenascin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Tenascin. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TN elisa kit

Principle of the assay: Tenascin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Tenascin antibody and an Tenascin-HRP conjugate. The assay sample and buffer are incubated together with Tenascin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Tenascin concentration since Tenascin from samples and Tenascin-HRP conjugate compete for the anti-Tenascin antibody binding site. Since the number of sites is limited, as more sites are occupied by Tenascin from the sample, fewer sites are left to bind Tenascin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tenascin concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TN elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 292826
• NCBI GeneID: 3371
• Molecular Weight: 171,360 Da
• NCBI Official Full Name: tenascin, partial
• NCBI Official Synonym Full Names: tenascin C
• NCBI Official Symbol: TNC
• NCBI Official Synonym Symbols: GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• NCBI Protein Information: tenascin; GP 150-225; cytotactin; neuronectin; myotendinous antigen; hexabrachion (tenascin); tenascin-C isoform 14/AD1/16; tenascin-C additional domain 1; deafness, autosomal dominant 56; glioma-associated-extracellular matrix antigen
• UniProt Protein Name: Tenascin
• Protein Family: Tenascin
• UniProt Gene Name: TNC
• UniProt Synonym Gene Names: HXB; TN; TN-C
• UniProt Entry Name: TENA_HUMAN

NCBI Description

This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

Uniprot Description

TNC: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. Belongs to the tenascin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular matrix; extracellular space; focal adhesion; membrane; extracellular region; basement membrane

Molecular Function: syndecan binding

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; response to ethanol; wound healing; response to mechanical stimulus; positive regulation of cell proliferation; response to wounding; negative regulation of cell adhesion; cell adhesion; axon regeneration in the peripheral nervous system; neuromuscular junction development; odontogenesis of dentine-containing teeth

Disease: Deafness, Autosomal Dominant 56