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Typical Testing Data/Standard Curve (for reference only)

Monkey Anti-amphiphysin Antibody ELISA Kit | AMPH elisa kit

Monkey Anti-amphiphysin Antibody ELISA Kit

Gene Names
AMPH; AMPH1
Reactivity
Monkey
Synonyms
Anti-amphiphysin Antibody; Monkey Anti-amphiphysin Antibody ELISA Kit; AMPH elisa kit
Ordering
For Research Use Only!
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
25-500ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This AMPH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MonkeyAMPH. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMPH elisa kit

Principle of the assay: AMPH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMPH antibody and an AMPH-HRP conjugate. The assay sample and buffer are incubated together with AMPH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPH concentration since AMPH from samples and AMPH-HRP conjugate compete for the anti-AMPH antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPH from the sample, fewer sites are left to bind AMPH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPH concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AMPH elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
273
Molecular Weight
71,929 Da
NCBI Official Full Name
amphiphysin
NCBI Official Synonym Full Names
amphiphysin
NCBI Official Symbol
AMPH
NCBI Official Synonym Symbols
AMPH1
NCBI Protein Information
amphiphysin; amphiphysin I; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
UniProt Protein Name
Amphiphysin
Protein Family
UniProt Gene Name
AMPH
UniProt Synonym Gene Names
AMPH1
UniProt Entry Name
AMPH_HUMAN

NCBI Description

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Uniprot Description

amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: synaptic vesicle; synaptic vesicle membrane; cell junction; actin cytoskeleton

Molecular Function: protein binding; phospholipid binding

Biological Process: synaptic transmission; synaptic vesicle endocytosis; endocytosis; learning

Research Articles on AMPH

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Product Notes

The Monkey AMPH amph (Catalog #AAA745086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745086 ELISA Kit recognizes Monkey AMPH. It is sometimes possible for the material contained within the vial of "Anti-amphiphysin Antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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