Canine Bone Morphogenetic Protein 10 ELISA Kit | BMP10 elisa kit

Canine Bone Morphogenetic Protein 10 ELISA Kit

Cat. Num. AAA742370

• Reactivity: Canine
• Synonyms: Bone Morphogenetic Protein 10; Canine Bone Morphogenetic Protein 10 ELISA Kit; BMP10 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of BMP-10. No significant cross-reactivity or interference between BMP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMP-10 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP10 elisa kit

Intended Uses: This BMP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BMP-10. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: BMP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BMP-10 antibody and an BMP-10-HRP conjugate. The assay sample and buffer are incubated together with BMP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-10 concentration since BMP-10 from samples and BMP-10-HRP conjugate compete for the anti-BMP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-10 from the sample, fewer sites are left to bind BMP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-10 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BMP10 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 7656928
• NCBI GeneID: 27302
• NCBI Accession #: NP_055297.1
• NCBI GenBank Nucleotide #: NM_014482.1
• Molecular Weight: 48,047 Da
• NCBI Official Full Name: bone morphogenetic protein 10 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: BMP10
• NCBI Protein Information: bone morphogenetic protein 10
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP10
• UniProt Synonym Gene Names: BMP-10
• UniProt Entry Name: BMP10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP10: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; Z disc

Molecular Function: receptor serine/threonine kinase binding; protein binding; telethonin binding; growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: adult heart development; extracellular matrix organization and biogenesis; atrial cardiac muscle morphogenesis; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; activin receptor signaling pathway; cardiac muscle cell proliferation; sarcomere organization; BMP signaling pathway; regulation of apoptosis; regulation of MAPKKK cascade; ventricular cardiac muscle morphogenesis; negative regulation of cell growth; cell adhesion; negative regulation of cell migration; positive regulation of cardiac muscle cell proliferation