Monkey Actinin Alpha 2 ELISA Kit | ACTN2 elisa kit

Monkey Actinin Alpha 2 ELISA Kit

Cat. Num. AAA742263

• Gene Names: ACTN2; CMD1AA
• Reactivity: Monkey
• Synonyms: Actinin Alpha 2; Monkey Actinin Alpha 2 ELISA Kit; ACTN2 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ACTN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ACTN2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTN2 elisa kit

Principle of the Assay: ACTN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTN2 antibody and an ACTN2-HRP conjugate. The assay sample and buffer are incubated together with ACTN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN2 concentration since ACTN2 from samples and ACTN2-HRP conjugate compete for the anti-ACTN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN2 from the sample, fewer sites are left to bind ACTN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ACTN2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4501893
• NCBI GeneID: 88
• NCBI Accession #: NP_001094.1
• NCBI GenBank Nucleotide #: NM_001103.3
• Molecular Weight: 103,920 Da
• NCBI Official Full Name: alpha-actinin-2 isoform 1
• NCBI Official Synonym Full Names: actinin, alpha 2
• NCBI Official Symbol: ACTN2
• NCBI Official Synonym Symbols: CMD1AA
• NCBI Protein Information: alpha-actinin-2; F-actin cross-linking protein; alpha-actinin skeletal muscle
• UniProt Protein Name: Alpha-actinin-2
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN2
• UniProt Entry Name: ACTN2_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42-q43

Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; dendritic spine; extracellular region; actin filament; pseudopodium; Z disc; cytosol; filopodium

Molecular Function: actin filament binding; protein dimerization activity; integrin binding; identical protein binding; LIM domain binding; protein binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity

Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion

Disease: Cardiomyopathy, Dilated, 1aa