Typical Testing Data/Standard Curve (for reference only)
Sheep Actinin Alpha 2 ELISA Kit | ACTN2 elisa kit
Sheep Actinin Alpha 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ACTN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ACTN2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ACTN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTN2 antibody and an ACTN2-HRP conjugate. The assay sample and buffer are incubated together with ACTN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN2 concentration since ACTN2 from samples and ACTN2-HRP conjugate compete for the anti-ACTN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN2 from the sample, fewer sites are left to bind ACTN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
Uniprot Description
ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 1q42-q43
Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; extracellular region; dendritic spine; actin filament; pseudopodium; cytosol; Z disc; filopodium
Molecular Function: integrin binding; actin filament binding; protein dimerization activity; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity
Biological Process: regulation of apoptosis; focal adhesion formation; synaptic transmission; platelet activation; regulation of membrane potential; platelet degranulation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; microspike biogenesis; blood coagulation; cell adhesion; muscle filament sliding; protein homotetramerization
Disease: Cardiomyopathy, Dilated, 1aa
Research Articles on ACTN2
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Product Notes
The Sheep ACTN2 actn2 (Catalog #AAA740663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740663 ELISA Kit recognizes Sheep ACTN2. It is sometimes possible for the material contained within the vial of "Actinin Alpha 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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