Porcine Apolipoprotein A5 ELISA Kit | APOA5 elisa kit

Porcine Apolipoprotein A5 ELISA Kit

Cat. Num. AAA741178

• Gene Names: APOA5; RAP3; APOAV
• Reactivity: Porcine
• Synonyms: Apolipoprotein A5; Porcine Apolipoprotein A5 ELISA Kit; APOA5 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This APOA5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine APOA5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA5 elisa kit

Principle of the assay: APOA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APOA5 antibody and an APOA5-HRP conjugate. The assay sample and buffer are incubated together with APOA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOA5 concentration since APOA5 from samples and APOA5-HRP conjugate compete for the anti-APOA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOA5 from the sample, fewer sites are left to bind APOA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOA5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOA5 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V; apo-AV; apolipoprotein A5; regeneration-associated protein 3
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; UNQ411/PRO773; Apo-AV; ApoA-V
• UniProt Entry Name: APOA5_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; cholesterol efflux; lipoprotein metabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; lipid transport; cholesterol biosynthetic process; positive regulation of receptor-mediated endocytosis; phosphatidylcholine metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; regulation of cholesterol absorption; phospholipid efflux; tissue regeneration; acylglycerol homeostasis; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity

Disease: Hypertriglyceridemia, Familial; Hyperlipoproteinemia, Type V