Canine Acylation Stimulating Protein ELISA Kit | ASP elisa kit

Canine Acylation Stimulating Protein ELISA Kit

Cat. Num. AAA741121

• Gene Names: CPAMD8; VIP; K-CAP
• Reactivity: Canine
• Synonyms: Acylation Stimulating Protein; Canine Acylation Stimulating Protein ELISA Kit; ASP elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ASP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ASP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASP elisa kit

Principle of the assay: ASP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ASP antibody and an ASP-HRP conjugate. The assay sample and buffer are incubated together with ASP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASP concentration since ASP from samples and ASP-HRP conjugate compete for the anti-ASP antibody binding site. Since the number of sites is limited, as more sites are occupied by ASP from the sample, fewer sites are left to bind ASP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ASP elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 118600977
• NCBI GeneID: 27151
• NCBI Accession #: NP_056507.2
• NCBI GenBank Nucleotide #: NM_015692.2
• Molecular Weight: 199,171 Da
• NCBI Official Full Name: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
• NCBI Official Synonym Full Names: C3 and PZP-like, alpha-2-macroglobulin domain containing 8
• NCBI Official Symbol: CPAMD8
• NCBI Official Synonym Symbols: VIP; K-CAP
• NCBI Protein Information: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; alpha-2 macroglobulin family protein VIP
• UniProt Protein Name: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
• Protein Family: Ancylostoma secreted protein
• UniProt Gene Name: CPAMD8
• UniProt Synonym Gene Names: KIAA1283
• UniProt Entry Name: CPMD8_HUMAN

NCBI Description

CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM 103950) family of proteins, which are involved in innate immunity and damage control. Complement components recognize and eliminate pathogens by direct binding or by mediating opsonization/phagocytosis and intracellular killing, and A2M is a broad-spectrum protease inhibitor (Li et al., 2004 [PubMed 15177561]).[supplied by OMIM, Mar 2008]

Uniprot Description

CPAMD8: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Up-regulated by IL1B/interleukin-1 beta and IL6/interleukin-6. Interacts with heparin. 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular space; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity