Porcine Tolloid Like Protein 1 ELISA Kit | TLL1 elisa kit

Porcine Tolloid Like Protein 1 ELISA Kit

Cat. Num. AAA738245

• Gene Names: TLL1; TLL; ASD6
• Reactivity: Porcine
• Synonyms: Tolloid Like Protein 1; Porcine Tolloid Like Protein 1 ELISA Kit; TLL1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0mU/L.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLL1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This TLL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine TLL1. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY TLL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLL1 antibody and an TLL1-HRP conjugate. The assay sample and buffer are incubated together with TLL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLL1 concentration since TLL1 from samples and TLL1-HRP conjugate compete for the anti-TLL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLL1 from the sample, fewer sites are left to bind TLL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLL1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TLL1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 325652110
• NCBI GeneID: 7092
• NCBI Accession #: NP_001191689.1
• NCBI GenBank Nucleotide #: NM_001204760.1
• Molecular Weight: 44,186 Da
• NCBI Official Full Name: tolloid-like protein 1 isoform 2
• NCBI Official Synonym Full Names: tolloid-like 1
• NCBI Official Symbol: TLL1
• NCBI Official Synonym Symbols: TLL; ASD6
• NCBI Protein Information: tolloid-like protein 1
• UniProt Protein Name: Tolloid-like protein 1
• Protein Family: Tolloid-like protein
• UniProt Gene Name: TLL1
• UniProt Synonym Gene Names: TLL
• UniProt Entry Name: TLL1_HUMAN

NCBI Description

This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

TLL1: Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. Defects in TLL1 are the cause of atrial septal defect type 6 (ASD6). A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the peptidase M12A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 4q32-q33

Cellular Component: extracellular region

Molecular Function: collagen binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; cell differentiation; skeletal development

Disease: Atrial Septal Defect 6