Goat GATA Binding Protein 3 ELISA Kit | GATA3 elisa kit

Goat GATA Binding Protein 3 ELISA Kit

Cat. Num. AAA737169

• Gene Names: GATA3; HDR; HDRS
• Reactivity: Goat
• Synonyms: GATA Binding Protein 3; Goat GATA Binding Protein 3 ELISA Kit; GATA3 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GATA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat GATA3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA3 elisa kit

Principle of the assay: GATA3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GATA3. Standards or samples are then added to the microtiter plate wells and GATA3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GATA3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GATA3 are added to each well to “sandwich” the GATA3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GATA3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GATA3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 55664494
• NCBI GeneID: 2625
• UniProt Accession #: P23771; Q5VWG7; Q5VWG8; Q96J16
• Molecular Weight: 47,916 Da
• NCBI Official Full Name: GATA binding protein 3
• NCBI Official Synonym Full Names: GATA binding protein 3
• NCBI Official Symbol: GATA3
• NCBI Official Synonym Symbols: HDR; HDRS
• NCBI Protein Information: trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3
• UniProt Protein Name: Trans-acting T-cell-specific transcription factor GATA-3
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA3
• UniProt Entry Name: GATA3_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Uniprot Description

GATA3: a transcriptional regulator central in Th2 cell differentiation. Inhibits breast cancer growth and pulmonary breast cancer metastasis. Represses INK4C transcription, constrains luminal progenitor cell expansion, and suppresses luminal tumorigenesis in the mammary gland. High GATA3 and low INK4C expression predicts a favorable patient outcome in luminal A type breast tumors. IFN-lambda1 (IL-29) inhibits GATA3 expression and suppresses Th2 responses in human T cells. GATA3 haploinsufficiency leads to HDR (hypoparathyroidism, deafness, and renal dysplasia) syndrome. Two alternatively spliced human isoforms have been described.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; zinc ion binding; interleukin-2 receptor binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: developmental growth; positive regulation of transcription, DNA-dependent; cell maturation; ear development; cell fate determination; uterus development; T cell receptor signaling pathway; negative regulation of interleukin-2 production; post-embryonic development; norepinephrine biosynthetic process; regulation of neuron apoptosis; anatomical structure formation; erythrocyte differentiation; mesonephros development; kidney development; regulation of cytokine biosynthetic process; response to drug; thymic T cell selection; anatomical structure morphogenesis; inner ear morphogenesis; positive regulation of signal transduction; embryonic hemopoiesis; response to virus; negative regulation of fat cell differentiation; parathyroid gland development; negative regulation of mammary gland epithelial cell proliferation; response to ethanol; positive regulation of T cell differentiation; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; pro-T cell differentiation; negative regulation of transcription, DNA-dependent; regulation of CD4-positive, alpha beta T cell differentiation; transcription from RNA polymerase II promoter; lens development in camera-type eye; axon guidance; phosphoinositide 3-kinase cascade; TOR signaling pathway; neuron migration; defense response; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of cell cycle; positive regulation of interleukin-4 production; negative regulation of cell proliferation; sympathetic nervous system development; negative regulation of interferon-gamma production; response to gamma radiation; type IV hypersensitivity; pharyngeal system development; thymus development; in utero embryonic development; male gonad development; humoral immune response; positive regulation of protein kinase B signaling cascade; negative regulation of inflammatory response; innate immune response; regulation of histone H3-K4 methylation; T-helper 2 cell differentiation; blood coagulation

Disease: Hypoparathyroidism, Sensorineural Deafness, And Renal Disease