Typical Testing Data/Standard Curve
Bovine Myoglobin ELISA Kit | Mb elisa kit
Bovine Myoglobin ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||Myoglobin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Myoglobin antibody and an Myoglobin-HRP conjugate. The assay sample and buffer are incubated together with Myoglobin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Myoglobin concentration since Myoglobin from samples and Myoglobin-HRP conjugate compete for the anti-Myoglobin antibody binding site. Since the number of sites is limited, as more sites are occupied by Myoglobin from the sample, fewer sites are left to bind Myoglobin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Myoglobin concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.
Protein type: Carrier
Chromosomal Location of Human Ortholog: 22q13.1
Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity
Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hypoxia; brown fat cell differentiation; response to hormone stimulus; enucleate erythrocyte differentiation
Research Articles on Mb
Similar Products
Product Notes
The Bovine Mb mb (Catalog #AAA735687) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735687 ELISA Kit recognizes Bovine Mb. It is sometimes possible for the material contained within the vial of "Myoglobin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
