Principle of the Assay: PGE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGE2 antibody and an PGE2-HRP conjugate. The assay sample and buffer are incubated together with PGE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE2 concentration since PGE2 from samples and PGE2-HRP conjugate compete for the anti-PGE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PGE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PGE2 antibody and an PGE2-HRP conjugate. The assay sample and buffer are incubated together with PGE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE2 concentration since PGE2 from samples and PGE2-HRP conjugate compete for the anti-PGE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.