Rabbit anti-Human ErbB4 Polyclonal Antibody | anti-ErbB4 antibody

ErbB4, NT (Receptor Tyrosine-protein Kinase erbB-4, Proto-oncogene-like Protein c-ErbB-4, p180erbB4, Tyrosine Kinase-type Cell Surface Receptor HER4, ERBB4) (Azide free) (HRP)

Cat. Num. AAA6474794

• Gene Names: ERBB4; HER4; p180erbB4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ErbB4; Polyclonal Antibody; NT (Receptor Tyrosine-protein Kinase erbB-4; Proto-oncogene-like Protein c-ErbB-4; p180erbB4; Tyrosine Kinase-type Cell Surface Receptor HER4; ERBB4) (Azide free) (HRP); anti-ErbB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ErbB4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ErbB4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 25-55 in the N-terminal region of human ErbB4, UniProt Accession #Q15303. Species sequence homology: mouse, rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL60 cell lysate usingMBS625936. ErbB4 (Arrow) was detected usingMBS625936. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue usingMBS625936, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625936 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-ErbB4 antibody

Antibodies; Oncoproteins

References

1. Cheng, Q.C., et al., J. Biol. Chem. 278(40):38421-38427 (2003). 2. Komuro, A., et al., J. Biol. Chem. 278(35):33334-33341 (2003). 3. Williams, E.E., et al., Cancer Lett. 192(1):67-74 (2003). 4. Thomas, C.Y., et al., Int. J. Cancer 104(1):19-27 (2003). 5. Ni, C.Y., et al., J. Biol. Chem. 278(7):4561-4565 (2003). 6.Gang Xu, et al. Circ. Res. published 30 July 2009, 10.1161/CIRCRESAHA.109.193870. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 110825958
• NCBI GeneID: 2066
• NCBI Accession #: NP_001036064.1
• NCBI GenBank Nucleotide #: NM_001042599.1
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 146,808 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-2
• NCBI Official Synonym Full Names: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: nucleoplasm; mitochondrion; basolateral plasma membrane; mitochondrial matrix; plasma membrane; extracellular region; integral to membrane; cytosol; nucleus; receptor complex

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; cell fate commitment; phosphoinositide-mediated signaling; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of protein amino acid phosphorylation; mitochondrial fragmentation during apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Amyotrophic Lateral Sclerosis 19