Canine Cyclooxygenase 2 ELISA Kit | COX2 elisa kit
Canine Cyclooxygenase 2 ELISA Kit
Principle of the Assay: COX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX2 antibody and an COX2-HRP conjugate. The assay sample and buffer are incubated together with COX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX2 concentration since COX2 from samples and COX2-HRP conjugate compete for the anti-COX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by COX2 from the sample, fewer sites are left to bind COX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Uniprot Description
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.99.1
Chromosomal Location of Human Ortholog: 1q25.2-q25.3
Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; protein homodimerization activity; enzyme binding; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding
Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; decidualization; sensory perception of pain; prostaglandin biosynthetic process; negative regulation of synaptic transmission, dopaminergic; positive regulation of synaptic transmission, glutamatergic; bone mineralization; response to estradiol stimulus; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; regulation of blood pressure; negative regulation of smooth muscle contraction; arachidonic acid metabolic process; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; water-soluble vitamin metabolic process; response to fructose stimulus; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; hair cycle; prostaglandin metabolic process; response to manganese ion; ovulation; NAD metabolic process; regulation of inflammatory response; positive regulation of fever; positive regulation of vasoconstriction; brown fat cell differentiation; response to oxidative stress; cell motility; embryo implantation