Mouse Macrophage chemotactic factor ELISA Kit | MCF elisa kit

Mouse Macrophage chemotactic factor ELISA Kit

Cat. Num. AAA732650

• Reactivity: Mouse
• Synonyms: Macrophage chemotactic factor; Mouse Macrophage chemotactic factor ELISA Kit; MCF elisa kit

• Reactivity: Mouse

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCF elisa kit

Intended Uses: This MDC ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of MDCin the sample, this MDCELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus MDCconcentration. The concentration of MDCin the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This MDCenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forMDC. Standards or samples are then added to the microtiter plate wells and MDCif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofMDCpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MDCare added to each well to "sandwich" the MDCimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain MDCand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 484255
• Molecular Weight: 57,911 Da
• NCBI Official Full Name: MDC protein
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 11
• UniProt Gene Name: ADAM11
• UniProt Synonym Gene Names: MDC; ADAM 11; MDC
• UniProt Entry Name: ADA11_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. [provided by RefSeq, Dec 2014]

Uniprot Description

ADAM11: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: extracellular matrix; plasma membrane; integral to membrane

Molecular Function: integrin binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: integrin-mediated signaling pathway; proteolysis