Mouse Macrophage Derived Chemokine (MDC) ELISA Kit | MDC elisa kit

Mouse Macrophage Derived Chemokine (MDC) ELISA Kit

Cat. Num. AAA454691

• Gene Names: Adam11; Mdc; AW060611
• Reactivity: Mouse
• Synonyms: Macrophage Derived Chemokine (MDC); Mouse Macrophage Derived Chemokine (MDC) ELISA Kit; CCL22; ABCD1; DC/B-CK; SCYA22; STCP1; Chemokine C-C-Motif Ligand 22; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22; Stimulated T-Cell Chemotactic Protein 1; MDC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MDC. No significant cross-reactivity or interference between MDC and analogues was observed.
• Sequence Length: 773

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.8pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MDC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MDC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MDC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MDC in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MDC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MDC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160707883
• NCBI GeneID: 11488
• NCBI Accession #: NP_033743.2
• NCBI GenBank Nucleotide #: NM_009613.3
• UniProt Accession #: Q9R1V4; A2AUA8
• Molecular Weight: 84,134 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 11 isoform 2 preproprotein
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 11
• NCBI Official Symbol: Adam11
• NCBI Official Synonym Symbols: Mdc; AW060611
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 11
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 11
• UniProt Gene Name: Adam11
• UniProt Synonym Gene Names: Mdc; ADAM 11; MDC

NCBI Description

This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit defects in spatial learning, motor coordination and altered perception of pain. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, May 2016]

Uniprot Description

Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein.