Typical Testing Data/Standard Curve (for reference only)
Human Carbonic Anhydrase 6, CA 6 ELISA Kit | CA6 elisa kit
Human Carbonic Anhydrase 6, CA 6 ELISA Kit
Gene Names
CA6; CA-VI; GUSTIN
Reactivity
Human
Synonyms
Carbonic Anhydrase 6; CA 6; Human Carbonic Anhydrase 6; CA 6 ELISA Kit; CA6 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CA6. No significant cross-reactivity or interference between CA6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CA6 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CA6 elisa kit
Intended Uses: This CA6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CA6. This ELISA kit for research use only!
Principle of the Assay: CA6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CA6 antibody and an CA6-HRP conjugate. The assay sample and buffer are incubated together with CA6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA6 concentration since CA6 from samples and CA6-HRP conjugate compete for the anti-CA6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA6 from the sample, fewer sites are left to bind CA6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CA6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CA6 antibody and an CA6-HRP conjugate. The assay sample and buffer are incubated together with CA6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA6 concentration since CA6 from samples and CA6-HRP conjugate compete for the anti-CA6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA6 from the sample, fewer sites are left to bind CA6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA6 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CA6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,687 Da
NCBI Official Full Name
carbonic anhydrase 6 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase VI
NCBI Official Symbol
CA6
NCBI Official Synonym Symbols
CA-VI; GUSTIN
NCBI Protein Information
carbonic anhydrase 6; carbonate dehydratase VI; salivary carbonic anhydrase; secreted carbonic anhydrase
UniProt Protein Name
Carbonic anhydrase 6
Protein Family
UniProt Gene Name
CA6
UniProt Synonym Gene Names
CA-VI
UniProt Entry Name
CAH6_HUMAN
NCBI Description
The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
CA6: Reversible hydration of carbon dioxide. Its role in saliva is unknown. Belongs to the alpha-carbonic anhydrase family.
Protein type: Secreted; Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p36.2
Cellular Component: extracellular space; cytoplasm; extracellular region
Molecular Function: carbonate dehydratase activity; zinc ion binding
Biological Process: bicarbonate transport; one-carbon compound metabolic process; detection of chemical stimulus involved in sensory perception of bitter taste
Research Articles on CA6
Similar Products
Product Notes
The Human CA6 ca6 (Catalog #AAA7251144) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7251144 ELISA Kit recognizes Human CA6. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase 6, CA 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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