Human 26S proteasome non ATPase regulatory subunit 1 (PSMD1) ELISA Kit | PSMD1 elisa kit

Human 26S proteasome non ATPase regulatory subunit 1 (PSMD1) ELISA Kit

Cat. Num. AAA7249605

• Gene Names: PSMD1; S1; P112; Rpn2
• Reactivity: Human
• Synonyms: 26S proteasome non ATPase regulatory subunit 1 (PSMD1); Human 26S proteasome non ATPase regulatory subunit 1 (PSMD1) ELISA Kit; PSMD1 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD1 elisa kit

Intended Uses: This PSAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PSAP. This ELISA kit for research use only!

Principle of the Assay: PSAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PSAP antibody and an PSAP-HRP conjugate. The assay sample and buffer are incubated together with PSAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSAP concentration since PSAP from samples and PSAP-HRP conjugate compete for the anti-PSAP antibody binding site. Since the number of sites is limited, as more sites are occupied by PSAP from the sample, fewer sites are left to bind PSAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSAP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PSMD1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 25777600
• NCBI GeneID: 5707
• NCBI Accession #: NP_002798.2
• NCBI GenBank Nucleotide #: NM_002807.3
• Molecular Weight: 102,258 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 1 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
• NCBI Official Symbol: PSMD1
• NCBI Official Synonym Symbols: S1; P112; Rpn2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 1; 26S proteasome subunit p112; 26S proteasome regulatory subunit S1; 26S proteasome regulatory subunit RPN2
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 1
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD1
• UniProt Entry Name: PSMD1_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2010]

Uniprot Description

PSMD1: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Interacts with ADRM1. Belongs to the proteasome subunit S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; cytosol

Molecular Function: enzyme regulator activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis