Typical Testing Data/Standard Curve (for reference only)
Human Cocaine esterase (CES2) ELISA Kit | CES2 elisa kit
Human Cocaine esterase (CES2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CES2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CES2 antibody and an CES2-HRP conjugate. The assay sample and buffer are incubated together with CES2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CES2 concentration since CES2 from samples and CES2-HRP conjugate compete for the anti-CES2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CES2 from the sample, fewer sites are left to bind CES2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CES2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]
Uniprot Description
CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.1.84; EC 3.1.1.56; Xenobiotic Metabolism - drug metabolism - other enzymes; Endoplasmic reticulum; EC 3.1.1.1; Hydrolase
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum
Molecular Function: methylumbelliferyl-acetate deacetylase activity
Biological Process: catabolic process
Research Articles on CES2
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Product Notes
The Human CES2 ces2 (Catalog #AAA7245485) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7245485 ELISA Kit recognizes Human CES2. It is sometimes possible for the material contained within the vial of "Cocaine esterase (CES2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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