GuineaPig Acetylcholine Receptor Antibody (AChR) ELISA Kit | AChR elisa kit

Guinea Pig Anti-Acetylcholine Receptor Antibody (Anti-AChR) ELISA Kit

Cat. Num. AAA724420

• Gene Names: CHRND; ACHRD; CMS2A; FCCMS; SCCMS
• Reactivity: GuineaPig
• Synonyms: Acetylcholine Receptor Antibody (AChR); Guinea Pig Anti-Acetylcholine Receptor Antibody (Anti-AChR) ELISA Kit; Anti-Acetylcholine Receptor Antibody (Anti-AChR); Anti-Acetylcholine Receptor Antibody ELISA Kit (Anti-AChR) (Guinea Pig); AChR elisa kit

• Reactivity: GuineaPig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This BG ACHRAB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig ACHRAB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AChR elisa kit

Principle of the Assay: ACHRAB ELISA kit applies the competitive enzyme immunoassay technique utilizing Acetylcholine Receptor antigen and an ACHRAB-HRP conjugate. The assay sample and buffer are incubated together with ACHRAB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACHRAB concentration since ACHRAB from samples and ACHRAB-HRP conjugate compete for the Acetylcholine Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by ACHRAB from the sample, fewer sites are left to bind ACHRAB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACHRAB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AChR elisa kit

Guinea Pig ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 297402
• NCBI GeneID: 1144
• UniProt Accession #: Q07001; Q52LH4; A8K661; B4DT92
• Molecular Weight: 58,895 Da
• NCBI Official Full Name: acetylcholine receptor delta subunit
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, delta (muscle)
• NCBI Official Symbol: CHRND
• NCBI Official Synonym Symbols: ACHRD; CMS2A; FCCMS; SCCMS
• NCBI Protein Information: acetylcholine receptor subunit delta; acetylcholine receptor, nicotinic, delta (muscle); cholinergic receptor, nicotinic, delta polypeptide
• UniProt Protein Name: Acetylcholine receptor subunit delta
• UniProt Gene Name: CHRND
• UniProt Synonym Gene Names: ACHRD
• UniProt Entry Name: ACHD_HUMAN

NCBI Description

The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL), congenital myasthenic syndrome slow-channel type (SCCMS), and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure: Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Involvement in disease: Multiple pterygium syndrome, lethal type (LMPS) [MIM:253290]: Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11Myasthenic syndrome, congenital, slow-channel (SCCMS) [MIM:601462]: A common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. Congenital myasthenic syndrome slow-channel type is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6 Ref.7Myasthenic syndrome, congenital, fast-channel (FCCMS) [MIM:608930]: A congenital myasthenic syndrome characterized by kinetic abnormalities of the AChR. Due in most cases to mutations that decrease activity of the AChR by slowing the rate of opening of the receptor channel, speeding the rate of closure of the channel, or decreasing the number of openings of the channel during ACh occupancy. The result is failure to achieve threshold depolarization of the endplate and consequent failure to fire an action potential.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.9

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Delta/CHRND sub-subfamily. [View classification]