Human Caspase 9 (CASP9) ELISA Kit | CASP9 elisa kit

Human Caspase 9 (CASP9) ELISA Kit

Cat. Num. AAA723796

• Gene Names: CASP9; MCH6; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
• Reactivity: Human
• Synonyms: Caspase 9 (CASP9); Human Caspase 9 (CASP9) ELISA Kit; Human Caspase 9 ELISA Kit (CASP9); Caspase 9 ELISA Kit (CASP9) (Human); CASP9 elisa kit

• Reactivity: Human

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP9 elisa kit

Intended Uses: This CASP9 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCASP9 in the sample, thisCASP9 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCASP9 concentration. The concentration ofCASP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This CASP9 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCASP9. Standards or samples are then added to the microtiter plate wells andCASP9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCASP9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCASP9 are added to each well to "sandwich" theCASP9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCASP9 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for CASP9 elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220.2
• NCBI GenBank Nucleotide #: NM_001229.3
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; Q59GG2; Q5JRU2; Q5JRU8; Q5UGI1; Q6EV95
• Molecular Weight: 46,281 Da
• NCBI Official Full Name: caspase-9 isoform alpha preproprotein
• NCBI Official Synonym Full Names: caspase 9, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
• NCBI Protein Information: caspase-9; OTTHUMP00000002322; OTTHUMP00000002323; OTTHUMP00000002324; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq]

Uniprot Description

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Catalytic activity: Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. Interacts (inactive form) with EFHD2. Interacts with HAX1. Ref.13 Ref.15 Ref.16

Tissue specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. Ref.13

Developmental stage: Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. Ref.13

Post-translational modification: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

Sequence similarities: Belongs to the peptidase C14A family.Contains 1 CARD domain.