Typical Testing Data/Standard Curve (for reference only)
Human F box/LRR repeat protein 2 (FBXL2) ELISA Kit | FBXL2 elisa kit
Human F box/LRR repeat protein 2 (FBXL2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FBXL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FBXL2 antibody and an FBXL2-HRP conjugate. The assay sample and buffer are incubated together with FBXL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXL2 concentration since FBXL2 from samples and FBXL2-HRP conjugate compete for the anti-FBXL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXL2 from the sample, fewer sites are left to bind FBXL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXL2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
Uniprot Description
FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
Protein type: Ubiquitin ligase
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: membrane; cytoplasm; SCF ubiquitin ligase complex
Molecular Function: calmodulin binding; protein binding; ubiquitin-protein ligase activity; protein phosphatase binding
Biological Process: protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; viral reproduction; regulation of phosphoinositide 3-kinase cascade; protein ubiquitination; protein modification process; regulation of autophagy; proteolysis
Research Articles on FBXL2
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Product Notes
The Human FBXL2 fbxl2 (Catalog #AAA7226859) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7226859 ELISA Kit recognizes Human FBXL2. It is sometimes possible for the material contained within the vial of "F box/LRR repeat protein 2 (FBXL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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