Goat FBL2/FBXL2 Polyclonal Antibody | anti-FBXL2 antibody

Goat anti-FBL2/FBXL2 Antibody

Cat. Num. AAA420664

• Gene Names: FBXL2; FBL2; FBL3
• Reactivity: Tested: Human, Rat; Expected from sequence similarity: Human, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FBL2/FBXL2; Polyclonal Antibody; Goat anti-FBL2/FBXL2 Antibody; FBXL2; FBL2; F-box and leucine-rich repeat protein 2; FBL3; DKFZP564P0622; F-box protein containing leucine-rich repeats; FBXL2 antibody; FBL2 antibody; F-box and leucine-rich repeat protein 2 antibody; FBL3 antibody; DKFZP564P0622 antibody; F-box protein containing leucine-rich repeats antibody; FBL2 / FBXL2; anti-FBXL2 antibody

• Host: Goat
• Reactivity: Tested: Human, Rat; Expected from sequence similarity: Human, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: VFSNNDEGLINKK
• Sequence Length: 423

• Applicable Applications for anti-FBXL2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration, 5-10ug/ml.; Western Blot: Approx 48kDa band observed in Human Brain (Frontal Cortex) lysates and approx 50kDa band observed in Rat Brain lysates (calculated MW of 47.1kDa according to Human NP_036289.2, and 46.9kDa according to Rat XP_343496.4). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence VFSNNDEGLINKK-C, from the N Terminus of the protein sequence according to NP_036289.2.
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Frontal Cortex (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Immunohistochemistry (IHC)

((10ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 16306582
• NCBI GeneID: 25827
• NCBI Accession #: NP_036289.2
• NCBI GenBank Nucleotide #: NM_012157.2
• Molecular Weight: 39,613 Da
• NCBI Official Full Name: F-box and leucine-rich repeat protein 2
• NCBI Official Synonym Full Names: F-box and leucine-rich repeat protein 2
• NCBI Official Symbol: FBXL2
• NCBI Official Synonym Symbols: FBL2; FBL3
• NCBI Protein Information: F-box/LRR-repeat protein 2
• UniProt Protein Name: F-box/LRR-repeat protein 2
• Protein Family: F-box/LRR-repeat protein
• UniProt Gene Name: FBXL2
• UniProt Entry Name: FBXL2_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.

Protein type: Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: cytoplasm; membrane; SCF ubiquitin ligase complex

Molecular Function: calmodulin binding; protein binding; protein phosphatase binding; ubiquitin-protein ligase activity

Biological Process: protein modification process; protein monoubiquitination; protein ubiquitination; proteolysis; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; viral reproduction