Rabbit anti-Human p40 phox Polyclonal Antibody | anti-p40-phox antibody

p40 phox, CT (p40phox, p40-phox, Neutrophil Cytosol Factor 4, Neutrophil Cytosolic Factor 4, NCF4, NCF-4, MGC3810, Neutrophil NADPH Oxidase Factor 4, SH3 and PX Domain-containing Protein 4, SH3PXD4) (MaxLight 650)

Cat. Num. AAA6491402

• Gene Names: NCF4; NCF; CGD3; P40PHOX; SH3PXD4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: p40 phox; Polyclonal Antibody; CT (p40phox; p40-phox; Neutrophil Cytosol Factor 4; Neutrophil Cytosolic Factor 4; NCF4; NCF-4; MGC3810; Neutrophil NADPH Oxidase Factor 4; SH3 and PX Domain-containing Protein 4; SH3PXD4) (MaxLight 650); 40kDa; anti-p40-phox antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NCF4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-p40-phox antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NCF4 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NCF4 antibody (C-term) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NCF4 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-p40-phox antibody

Antibodies; Enzymes; Oxidase

References

1. Glas, J., Seiderer, J. Am. J. Gastroenterol. 104(3), 665-672 (2009). 2. Honbou, K. Seikagaku 80(8), 743-747 (2008). 3. Dusi, S., Donini, M. Biochem. J. 314(PT 2), 409-412 (1996). 4. Leto, T.L. Proc. Natl. Acad. Sci. USA 91(22), 10650-10654 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 47519798
• NCBI GeneID: 4689
• NCBI Accession #: NP_000622
• NCBI GenBank Nucleotide #: NM_000631.4
• UniProt Accession #: Q15080; O60808; Q86U56; Q9BU98; Q9NP45; A8K4F9
• Molecular Weight: 41.1 kDa (359aa) confirmed by MALDI-TOF
• NCBI Official Full Name: neutrophil cytosol factor 4 isoform 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 4
• NCBI Official Symbol: NCF4
• NCBI Official Synonym Symbols: NCF; CGD3; P40PHOX; SH3PXD4
• NCBI Protein Information: neutrophil cytosol factor 4
• UniProt Protein Name: Neutrophil cytosol factor 4
• UniProt Gene Name: NCF4
• UniProt Synonym Gene Names: SH3PXD4; NCF-4; p40phox
• UniProt Entry Name: NCF4_HUMAN

NCBI Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: membrane; endosome membrane; cytosol; NADPH oxidase complex

Molecular Function: protein dimerization activity; protein binding; superoxide-generating NADPH oxidase activator activity; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of catalytic activity; interaction with host; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii