Principle of the Assay: NR2F2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NR2F2 antibody and an NR2F2-HRP conjugate. The assay sample and buffer are incubated together with NR2F2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NR2F2 concentration since NR2F2 from samples and NR2F2-HRP conjugate compete for the anti-NR2F2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NR2F2 from the sample, fewer sites are left to bind NR2F2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NR2F2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
Uniprot Description
NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.
Protein type: Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Ras; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: Golgi membrane; membrane; plasma membrane
Molecular Function: GTP binding; protein complex binding
Biological Process: epidermal growth factor receptor signaling pathway; regulation of long-term neuronal synaptic plasticity; negative regulation of skeletal muscle development; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; positive regulation of cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; visual learning; striated muscle cell differentiation; innate immune response; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration
Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Colorectal Cancer; Melanocytic Nevus Syndrome, Congenital; Melanosis, Neurocutaneous; Nevus, Epidermal