Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit | ILP2 elisa kit

Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit

Cat. Num. AAA722176

• Gene Names: BIRC8; ILP2; ILP-2; hILP2
• Reactivity: Human
• Synonyms: Inhibitor of apoptosis protein Like Protein2; Human Inhibitor of apoptosis protein Like Protein2 ELISA Kit; ILP2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This IPLP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IPLP-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ILP2 elisa kit

Priciple of the assay: IPLP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IPLP-2 antibody and an IPLP-2-HRP conjugate. The assay sample and buffer are incubated together with IPLP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IPLP-2 concentration since IPLP-2 from samples and IPLP-2-HRP conjugate compete for the anti-IPLP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IPLP-2 from the sample, fewer sites are left to bind IPLP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IPLP-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ILP2 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 15042064
• NCBI GeneID: 112401
• Molecular Weight: 27,089 Da
• NCBI Official Full Name: IAP-like protein 2
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 8
• NCBI Official Symbol: BIRC8
• NCBI Official Synonym Symbols: ILP2; ILP-2; hILP2
• NCBI Protein Information: baculoviral IAP repeat-containing protein 8; IAP-like protein 2; baculoviral IAP repeat-containing 8; inhibitor of apoptosis-like protein 2; testis-specific inhibitor of apoptosis
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 8
• Protein Family: Probable insulin-like peptide
• UniProt Gene Name: BIRC8
• UniProt Synonym Gene Names: ILP2; IAP-like protein 2; ILP-2
• UniProt Entry Name: BIRC8_HUMAN

Uniprot Description

BIRC8: Protects against apoptosis mediated by BAX. Belongs to the IAP family.

Protein type: Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: -

Cellular Component: spindle microtubule; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protease binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity

Biological Process: neuron apoptosis; protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of apoptosis