Human 26S proteasome non ATPase regulatory subunit 11 ELISA Kit | PSMD11 elisa kit

Human 26S proteasome non ATPase regulatory subunit 11 ELISA Kit

Cat. Num. AAA7218258

• Gene Names: PSMD11; S9; Rpn6; p44.5
• Reactivity: Human
• Synonyms: 26S proteasome non ATPase regulatory subunit 11; Human 26S proteasome non ATPase regulatory subunit 11 ELISA Kit; PSMD11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSMD11. No significant cross-reactivity or interference between PSMD11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSMD11 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD11 elisa kit

Intended Uses: This PSMD11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanPSMD11. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: PSMD11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PSMD11 antibody and an PSMD11-HRP conjugate. The assay sample and buffer are incubated together with PSMD11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSMD11 concentration since PSMD11 from samples and PSMD11-HRP conjugate compete for the anti-PSMD11 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSMD11 from the sample, fewer sites are left to bind PSMD11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSMD11 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PSMD11 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 394953908
• NCBI GeneID: 5717
• NCBI Accession #: NP_001257411.1
• NCBI GenBank Nucleotide #: NM_001270482.1
• Molecular Weight: 47,535 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 11
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• NCBI Official Symbol: PSMD11
• NCBI Official Synonym Symbols: S9; Rpn6; p44.5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit p44.5
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 11
• UniProt Gene Name: PSMD11
• UniProt Entry Name: PSD11_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; stem cell differentiation; proteasome assembly; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis