Typical Testing Data/Standard Curve (for reference only)
Mouse natriuretic peptide precursor C (NPPC) ELISA Kit | NPPC elisa kit
Mouse natriuretic peptide precursor C (NPPC) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NPPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NPPC antibody and an NPPC-HRP conjugate. The assay sample and buffer are incubated together with NPPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPPC concentration since NPPC from samples and NPPC-HRP conjugate compete for the anti-NPPC antibody binding site. Since the number of sites is limited, as more sites are occupied by NPPC from the sample, fewer sites are left to bind NPPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPPC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]
Uniprot Description
NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.
Protein type: Hormone; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: extracellular space; secretory granule
Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding
Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; negative regulation of DNA metabolic process; positive regulation of vasodilation; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size
Research Articles on NPPC
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Product Notes
The Mouse NPPC nppc (Catalog #AAA7217343) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217343 ELISA Kit recognizes Mouse NPPC. It is sometimes possible for the material contained within the vial of "natriuretic peptide precursor C (NPPC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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