Typical Testing Data/Standard Curve (for reference only)
Human Cyclin dependent kinase inhibitor 1C (CDKN1C) ELISA Kit | CDKN1C elisa kit
Human Cyclin dependent kinase inhibitor 1C (CDKN1C) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CDKN1C ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDKN1C antibody and an CDKN1C-HRP conjugate. The assay sample and buffer are incubated together with CDKN1C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDKN1C concentration since CDKN1C from samples and CDKN1C-HRP conjugate compete for the anti-CDKN1C antibody binding site. Since the number of sites is limited, as more sites are occupied by CDKN1C from the sample, fewer sites are left to bind CDKN1C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDKN1C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Uniprot Description
p57Kip2: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life. Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. Belongs to the CDI family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Cell cycle regulation; Nuclear receptor co-regulator; Tumor suppressor
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; nucleus
Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding
Biological Process: negative regulation of kinase activity; camera-type eye development; adrenal gland development; multicellular organism growth; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; uterus development; neuron maturation; negative regulation of phosphorylation; myeloid cell differentiation; negative regulation of transcription, DNA-dependent; cell cycle arrest; kidney development; skeletal development; negative regulation of epithelial cell proliferation; aging
Disease: Intrauterine Growth Retardation, Metaphyseal Dysplasia, Adrenal Hypoplasia Congenita, And Genital Anomalies; Beckwith-wiedemann Syndrome
Research Articles on CDKN1C
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Product Notes
The Human CDKN1C cdkn1c (Catalog #AAA7217208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217208 ELISA Kit recognizes Human CDKN1C. It is sometimes possible for the material contained within the vial of "Cyclin dependent kinase inhibitor 1C (CDKN1C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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