Mouse ATP-binding cassette sub-family A member 7 (ABCA7) ELISA Kit | ABCA7 elisa kit

Mouse ATP-binding cassette sub-family A member 7 (ABCA7) ELISA Kit

Cat. Num. AAA7211637

• Gene Names: ABCA7; ABCX; ABCA-SSN
• Reactivity: Mouse
• Synonyms: ATP-binding cassette sub-family A member 7 (ABCA7); Mouse ATP-binding cassette sub-family A member 7 (ABCA7) ELISA Kit; ABCA7 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA7 elisa kit

Intended Uses: This ABCA7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ABCA7. This ELISA kit for research use only!

Principle of the Assay: ABCA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCA7 antibody and an ABCA7-HRP conjugate. The assay sample and buffer are incubated together with ABCA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCA7 concentration since ABCA7 from samples and ABCA7-HRP conjugate compete for the anti-ABCA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCA7 from the sample, fewer sites are left to bind ABCA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCA7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ABCA7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 150417984
• NCBI GeneID: 10347
• NCBI Accession #: NP_061985.2
• NCBI GenBank Nucleotide #: NM_019112.3
• Molecular Weight: 218,497 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 7
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 7
• NCBI Official Symbol: ABCA7
• NCBI Official Synonym Symbols: ABCX; ABCA-SSN
• NCBI Protein Information: ATP-binding cassette sub-family A member 7; autoantigen SS-N; macrophage ABC transporter
• UniProt Protein Name: ATP-binding cassette sub-family A member 7
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA7
• UniProt Entry Name: ABCA7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA7: Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Belongs to the ABC transporter superfamily. ABCA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; Golgi apparatus; cell surface; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; endosome membrane; ATP-binding cassette (ABC) transporter complex; phagocytic cup

Molecular Function: apolipoprotein A-I receptor activity; phospholipid transporter activity; transporter activity; ATPase activity; ATP binding

Biological Process: phospholipid efflux; positive regulation of ATPase activity; negative regulation of amyloid precursor protein biosynthetic process; positive regulation of phagocytosis; cholesterol efflux; peptide cross-linking; phospholipid scrambling; transmembrane transport; negative regulation of ATPase activity; memory; phagocytosis