Typical Testing Data/Standard Curve (for reference only)
Rat Hormone Sensitive Lipase ELISA Kit | HSL elisa kit
Rat Hormone Sensitive Lipase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HSD17B13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HSD17B13 antibody and an HSD17B13-HRP conjugate. The assay sample and buffer are incubated together with HSD17B13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSD17B13 concentration since HSD17B13 from samples and HSD17B13-HRP conjugate compete for the anti-HSD17B13 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSD17B13 from the sample, fewer sites are left to bind HSD17B13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSD17B13 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
Uniprot Description
HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.
Protein type: EC 3.1.1.79; Lipase
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: lipid particle; caveola; cytosol
Molecular Function: hormone-sensitive lipase activity; triacylglycerol lipase activity; protein binding; protein kinase binding
Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process
Disease: Abdominal Obesity-metabolic Syndrome 4
Research Articles on HSL
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Product Notes
The Rat HSL lipe (Catalog #AAA720741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720741 ELISA Kit recognizes Rat HSL. It is sometimes possible for the material contained within the vial of "Hormone Sensitive Lipase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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