Related Product Information for CRH elisa kit
For Sample Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This CRH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CRH. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CRH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRH antibody and an CRH-HRP conjugate. The assay sample and buffer are incubated together with CRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRH concentration since CRH from samples and CRH-HRP conjugate compete for the anti-CRH antibody binding site. Since the number of sites is limited, as more sites are occupied by CRH from the sample, fewer sites are left to bind CRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRH concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CRH elisa kit
Product Notes
The Rabbit CRH (Catalog #
AAA720169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720169 ELISA Kit recognizes Rabbit CRH.
It is sometimes possible for the material contained within the vial of
"Corticotropin Releasing Hormone (CRH), ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
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responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
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of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
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