Rabbit anti-Human, Mouse ARRB2 Polyclonal Antibody | anti-ARRB2 antibody

ARRB2 (Arrestin beta-2, Beta-arrestin-2, ARB2, ARR2, BARR2) (FITC)

Cat. Num. AAA6407315

• Gene Names: ARRB2; ARB2; ARR2; BARR2
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: ARRB2; Polyclonal Antibody; ARRB2 (Arrestin beta-2; Beta-arrestin-2; ARB2; ARR2; BARR2) (FITC); anti-ARRB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ARRB2. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ARRB2 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:20-1:50; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ARRB2.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of RAW264.7 cell line, using ARRB2 antibody.)

Product Categories/Family for anti-ARRB2 antibody

Antibodies; Arrestin

NCBI and Uniprot Product Information

• NCBI GI #: 20141230
• NCBI GeneID: 409
• UniProt Accession #: P32121; Q0Z8D3; Q2PP19; Q6ICT3; Q8N7Y2; Q9UEQ6; B4DLW0; B5B0C0; B7WPL3; D3DTK2; H0Y688
• Molecular Weight: 409
• NCBI Official Full Name: Beta-arrestin-2
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: ARRB2
• NCBI Official Synonym Symbols: ARB2; ARR2; BARR2
• NCBI Protein Information: beta-arrestin-2; arrestin 3; arrestin beta-2
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB2
• UniProt Synonym Gene Names: ARB2; ARR2
• UniProt Entry Name: ARRB2_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: postsynaptic membrane; endocytic vesicle; basolateral plasma membrane; postsynaptic density; cytoplasm; plasma membrane; dendritic spine; coated pit; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: angiotensin receptor binding; protein domain specific binding; protein kinase B binding; type 2A serotonin receptor binding; follicle stimulating hormone receptor binding; alpha-1B adrenergic receptor binding; alpha-1A adrenergic receptor binding; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; protein complex binding; mitogen-activated protein kinase binding; type 1 angiotensin receptor binding; protein complex scaffold; D1 dopamine receptor binding; receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of receptor internalization; protein ubiquitination; negative regulation of caspase activity; adult walking behavior; protein transport; receptor internalization; transforming growth factor beta receptor signaling pathway; negative regulation of interleukin-6 production; detection of temperature stimulus involved in sensory perception of pain; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; platelet activation; negative regulation of interleukin-12 production; Notch signaling pathway; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of synaptic transmission, dopaminergic; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; positive regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of protein ubiquitination; brain development; positive regulation of calcium ion transport; blood coagulation