Rabbit NRPE1 Polyclonal Antibody | anti-NRPE1 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPE1 Polyclonal Antibody

• Gene Names: NRPD1B; ATNRPD1B; DEFECTIVE IN MERISTEM SILENCING 5; DMS5; DRD3; NRPE1; nuclear RNA polymerase D1B; T28M21.19; T28M21_19
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: NRPE1; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPE1 Polyclonal Antibody; NRPE1 Antibody; DNA-directed RNA polymerase V subunit 1; DNA-directed RNA polymerase D subunit 1b; AtNRPD1b; Nuclear RNA polymerase D 1b; DNA-directed RNA polymerase E subunit 1; Nuclear RNA polymerase E 1; EC 2.7.7.6; Protein DEFECTIVE IN MERISTEM SILENCING 5; Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3; Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1; RNA polymerase IV subunit 1; POL IV 1; NRPE1 DMS5 DRD3 NRPD1b RMD1 RPE1 At2g40030 T28M21.19; anti-NRPE1 antibody

• Host: Rabbit
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-NRPE1 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Arabidopsis thaliana NRPE1 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 79571777
• NCBI GeneID: 818591
• NCBI Accession #: NP_181532.2
• NCBI GenBank Nucleotide #: NM_129561.4
• UniProt Accession #: Q5D869; O04206; O04207; Q1L5X4
• Molecular Weight: 218,228 Da
• NCBI Official Full Name: nuclear RNA polymerase D1B
• NCBI Official Symbol: NRPD1B
• NCBI Official Synonym Symbols: ATNRPD1B; DEFECTIVE IN MERISTEM SILENCING 5; DMS5; DRD3; NRPE1; nuclear RNA polymerase D1B; T28M21.19; T28M21_19
• NCBI Protein Information: nuclear RNA polymerase D1B
• UniProt Protein Name: DNA-directed RNA polymerase V subunit 1
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: NRPE1
• UniProt Synonym Gene Names: DMS5; DRD3; NRPD1b; RMD1; RPE1; AtNRPD1b; Nuclear RNA polymerase D 1b; Nuclear RNA polymerase E 1; POL IV 1

NCBI Description

Encodes the unique largest subunit of nuclear DNA-dependent RNA polymerase V; homologous to budding yeast RPB1 and the E. coli RNA polymerase beta prime subunit. Required for normal RNA-directed DNA methylation at non-CG methylation sites and transgene silencing.

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA-directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Also required for full erasure of methylation when the RNA trigger is withdrawn. Seems also involved in the synthesis of short-interfering RNAs (siRNA). Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Involved in the maintenance of post-transcriptional RNA silencing.