Rabbit MACB Polyclonal Antibody | anti-MACB antibody

Rabbit anti-Escherichia coli (strain K12) MACB Polyclonal Antibody

• Gene Names: macB; ECK0870; JW0863; ybjZ
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: MACB; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) MACB Polyclonal Antibody; MACB Antibody; Macrolide export ATP-binding/permease protein MacB; EC 3.6.3.-; macB ybjZ b0879 JW0863; anti-MACB antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-MACB antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) macB protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 16128847
• NCBI GeneID: 945164
• NCBI Accession #: NP_415400.1
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P75831
• Molecular Weight: 70,702 Da
• NCBI Official Full Name: fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component
• NCBI Official Symbol: macB
• NCBI Official Synonym Symbols: ECK0870; JW0863; ybjZ
• NCBI Protein Information: fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component
• UniProt Protein Name: Macrolide export ATP-binding/permease protein MacB
• Protein Family: Macrolide export ATP-binding/permease protein
• UniProt Gene Name: macB
• UniProt Synonym Gene Names: ybjZ
• UniProt Entry Name: MACB_ECOLI

NCBI Description

MacABTolC exports 14-15 membered lactone macrolides, e.g. erythromycin. [More information is available at EcoGene: EG13695]. MacB, also known as YbjZ is a half-type ABC protein component of the macrolide efflux transporter complex, MacAB. [More information is available at EcoCyc: G6462].

Uniprot Description

Function: Part of the ABC transporter complex MacAB involved in macrolide export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation. Seems to confer resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones

Probable. Ref.1

Subunit structure: Homodimer

Probable.

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.1.

Domain: In MacB the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Miscellaneous: The complex MacAB-TolC (OMF) forms a single transport channel across the two membranes, allowing direct export of macrolide. This channel is involved in type 1 secretion system.

Sequence similarities: Belongs to the ABC transporter superfamily. Macrolide exporter (TC 3.A.1.122) family. [View classification]Contains 1 ABC transporter domain.