Immunofluorescence (IF)
(Immunofluorescence staining of SH-SY5Y cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Rabbit anti-Human ELF2 Polyclonal Antibody | anti-ELF2 antibody
Rabbit anti-Human ELF2 Polyclonal Antibody
Gene Names
ELF2; EU32; NERF; NERF-2; NERF-1A; NERF-1B; NERF-1a,b
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ELF2; Polyclonal Antibody; Rabbit anti-Human ELF2 Polyclonal Antibody; ETS-related transcription factor Elf-2 (E74-like factor 2) (New ETS-related factor); NERF; ELF2 Antibody; anti-ELF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid. Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-ELF2 antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:100-1:500
Immunogen Species
Human
Immunogen
Recombinant Human ETS-related transcription factor Elf-2 protein (1-353aa)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of SH-SY5Y cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of SH-SY5Y cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for anti-ELF2 antibody
Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.
Product Categories/Family for anti-ELF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,967 Da
NCBI Official Full Name
ETS-related transcription factor Elf-2 isoform 1
NCBI Official Synonym Full Names
E74-like factor 2 (ets domain transcription factor)
NCBI Official Symbol
ELF2
NCBI Official Synonym Symbols
EU32; NERF; NERF-2; NERF-1A; NERF-1B; NERF-1a,b
NCBI Protein Information
ETS-related transcription factor Elf-2; OTTHUMP00000164540; OTTHUMP00000164541; OTTHUMP00000220051; OTTHUMP00000220053; new Ets-related factor; ets family transcription factor ELF2C
UniProt Protein Name
ETS-related transcription factor Elf-2
Protein Family
UniProt Gene Name
ELF2
UniProt Synonym Gene Names
NERF
UniProt Entry Name
ELF2_HUMAN
Uniprot Description
Elf-2: Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter. Belongs to the ETS family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 4q28
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell differentiation; negative regulation of transcription, DNA-dependent
Research Articles on ELF2
Similar Products
Product Notes
The ELF2 elf2 (Catalog #AAA7137471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human ELF2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ELF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:100-1:500. Researchers should empirically determine the suitability of the ELF2 elf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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