Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, THP-1 whole cell lysate, U251 whole cell lysateAll lanes: CCHCR1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 100, 96 kDaObserved band size: 89 kDa)
Rabbit anti-Human CCHCR1 Polyclonal Antibody | anti-CCHCR1 antibody
Rabbit anti-Human CCHCR1 Polyclonal Antibody
Gene Names
CCHCR1; HCR; SBP; C6orf18
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
CCHCR1; Polyclonal Antibody; Rabbit anti-Human CCHCR1 Polyclonal Antibody; Coiled-coil alpha-helical rod protein 1 (Alpha-helical coiled-coil rod protein) (Putative gene 8 protein) (Pg8); C6orf18 HCR; CCHCR1 Antibody; anti-CCHCR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid. Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-CCHCR1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IHC: 1:100-1:500
IF: 1:100-1:500
IHC: 1:100-1:500
IF: 1:100-1:500
Immunogen Species
Human
Immunogen
Recombinant Human Coiled-coil alpha-helical rod protein 1 protein (281-500aa)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, THP-1 whole cell lysate, U251 whole cell lysateAll lanes: CCHCR1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 100, 96 kDaObserved band size: 89 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, THP-1 whole cell lysate, U251 whole cell lysateAll lanes: CCHCR1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 100, 96 kDaObserved band size: 89 kDa)
Immunohistochemistry (IHC)
(IHC image of diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image of diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with -IF at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with -IF at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for anti-CCHCR1 antibody
May be a regulator of keratinocyte proliferation or differentiation.
Product Categories/Family for anti-CCHCR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,671 Da
NCBI Official Full Name
coiled-coil alpha-helical rod protein 1 isoform 2
NCBI Official Synonym Full Names
coiled-coil alpha-helical rod protein 1
NCBI Official Symbol
CCHCR1
NCBI Official Synonym Symbols
HCR; SBP; C6orf18
NCBI Protein Information
coiled-coil alpha-helical rod protein 1; pg8; StAR-binding protein; putative gene 8 protein; alpha-helical coiled-coil rod protein; HCR (a-helix coiled-coil rod homologue)
UniProt Protein Name
Coiled-coil alpha-helical rod protein 1
Protein Family
UniProt Gene Name
CCHCR1
UniProt Synonym Gene Names
C6orf18; HCR; Pg8
UniProt Entry Name
CCHCR_HUMAN
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Product Notes
The CCHCR1 cchcr1 (Catalog #AAA7137137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human CCHCR1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCHCR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:100-1:500 IF: 1:100-1:500. Researchers should empirically determine the suitability of the CCHCR1 cchcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCHCR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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