Western Blot (WB)
(Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using AMPK a1 (EM1327) Mouse mAb diluted at 1:2000.)
Mouse AMPKalpha1 Monoclonal Antibody | anti-AMPKalpha1 antibody
AMPKalpha1 (9G3) Mouse mAb
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Synonyms
AMPKalpha1; Monoclonal Antibody; AMPKalpha1 (9G3) Mouse mAb; AAPK1; 5 AMP activated protein kinase alpha 1 catalytic subunit; PRKAA1; SNF1A; MGC33776; anti-AMPKalpha1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Form/Format
PBS with 0.02% sodium azide and 50% glycerol pH7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-AMPKalpha1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000-2,000
IHC: 1:100-200
IHC: 1:100-200
Species
Human
Immunogen
Recombinant Protein
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using AMPK a1 (EM1327) Mouse mAb diluted at 1:2000.)
Western Blot (WB)
(Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using AMPK a1 (EM1327) Mouse mAb diluted at 1:2000.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using AMPk a1(EM1327)Mouse mAb diluted at 1:200. )
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using AMPk a1(EM1327)Mouse mAb diluted at 1:200. )
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Skeletal Muscle Tissue using AMPk a1(EM1327)Mouse mAb diluted at 1:200.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Skeletal Muscle Tissue using AMPk a1(EM1327)Mouse mAb diluted at 1:200.)
Related Product Information for anti-AMPKalpha1 antibody
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, 2; beta1, 2; gamma1, 2, 3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,009 Da
NCBI Official Full Name
PRKAA1 protein
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
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Product Notes
The AMPKalpha1 prkaa1 (Catalog #AAA8808690) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AMPKalpha1 (9G3) Mouse mAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMPKalpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-2,000 IHC: 1:100-200. Researchers should empirically determine the suitability of the AMPKalpha1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPKalpha1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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